Part:BBa_K2683030

Peptidyl Prolyl Isomerase (PPiase) in pSB1C3

Peptidyl-prolyl isomerase (PPiase) is a protein that aids the isomerization of prolines in order to catalyze the addition proline amino acids onto the peptide chains from the aminoacyl-tRNA it is attached to [1]. This part has been codon optimized for use in E.coli , Contains a N-terminal his tag, and has a T7 promoter, medium strength RBS, and double terminator. This part was originally designed by the 2017 LEthbridge iGEm team (http://2017.igem.org/Team:Lethbridge).

This part was improved by placing it into the pSB1C3 plasmid.

Figure 1- An overexpression gel of PPiase protein run on a 12% SDS-PAGE. Samples were taken at point of induction and then in 1 hour increments thereafter. The induced proteins is expected to be seen at about 22kDa range

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]